Holy Trinity Churchyard was created in 1848 when the church was built. Previously it is understood to have been allotments. It is bounded largely by brick and brick and flint walls; those on the north side being Grade II listed. A number of trees were planted originally, notably three Cypress trees and silver birches, also limes, hornbeam and yews behind the church and two "Trees of Heaven", a whitebeam, copper beech, walnut and rowans in front.

Since 1995 the churchyard has been managed for wildlife. Although the management is not officially within the ambit of Henley Wildlife Group, the manager is a member of the Group which, thus, has an interest. Advice from the Berks, Bucks & Oxon Wildlife Trust indicated the need for little change from the existing mowing regime (largely dictated by available manpower!). Close cut areas are maintained next to the building, along the North boundary and to access paths. Other areas are cut less frequently and the remainder once a year in rotation, thus diversifying habitats for wildflowers, insects and animals.

A very chalky subsoil results in a rich flora. Floral surveys conducted by Roy Maycock for BBOWT in 1992 and by Linda Carter in 1999 listed 150 wildflowers and over 60 "species usually found near habitation"and cultivars. Two species of fungi, rare in Oxfordshire, have been found by Professor Richard Fortey. To mark the Millennium a small "quiet garden" was formed in an unconsecrated area, part of which is given over to wildflowers not otherwise present in the churchyard.

Consequent upon the rich flora, many species of butterfly are present; some 19 to 21 are regularly recorded in annual returns to Butterfly Conservation which advises this is one of the greatest number from churchyard returns in Berks, Bucks & Oxon.

Birdlife is also plentiful. Green and Greater spotted woodpeckers and Spotted flycatchers are seen as well as the now less common song thrush, also wrens and long tailed tits. Mammals are present including foxes, a badger, muntjac deer and hedgehogs.